Prolo Plus

Prolotherapy under ultrasound guidance

Prolotherapy is the simplest form of Regenerative Medicine. In 2009 and after long-term use and evaluation of all of the interventional procedures, medications and alternative medicine (eg acupuncture) for the treatment of pain, both in Greece and overseas, have chosen Prolotherapy as the baseline of our therapeutic approach in our clinic. It is an effective method for the treatment of the problem – not only symptomatic treatment – without substantial side effects.

What is prolotherapy

Where does the term come from

The term prolotherapy emanates from the English term proliferation therapy that means multiplicative treatment. The method has been shaped in its current form mainly by Hackett and Hemwall during the 1950’s in the US. It has been applied since 2005 from the doctors of Mayo Clinic, who characteristically report that “… contrary to the injections of cortisone, that offer provisional comfort, prolotherapy causes improvement of the tissue activating its rebirth”.

How it heals

The injections cause local stimulus which activates the natural mechanism of self-healing and leads to the generation of new conjunctive tissue (collagen), that is the structural component of the body. The new collagen surrounds the wounded tissue and thus hardens it. In regard to musculoskeletal problems, after the treatment, tendons, ligaments , and cartilage have been strengthened with new tissue and are more robust. In certain cases it is reported that they are hardened 40% more than the corresponding healthy ones. Thus, prolotherapy is not only pain relieving but therapeutic as well, as it restores the damaged tissue which caused pain.

Where it applies

How we apply prolotherapy in our pain clinic

As it has already been stated, the treatment is applied with a series of injections under ultrasound guidance in locations where damage is observed. However, our extensive experience in the treatment of pain, enables us to incorporate elements from other minimal invasive techniques during Prolotherapy sessions in order to achieve even better results. These techniques are: trigger point injections, neural prolotherapy, nerve release, central or peripheral nervous shut-off, perineural infiltrations, calcifications dissolution, and removal of debris.

The injections do not hurt much and are usually well received by most patients. However, If someone is too sensitive or an extensive treatment is to be performed, then prior to the treatment we provide local anesthesia to the locations that the injections are to be applied.

Ultrasound guidance

Prolotherapy may be performed under ultrasound guidance to achieve maximum results. Musculoskeletal Ultrasound allows us to guide the needle precisely into the human body and apply the injections to the exact location of the damage.

In addition, ultrasound enables us to compare the condition of tissues and components of the musculoskeletal system of the patient before the initiation of the treatments and during the implementation of the treatment plan. In this way, we are able to monitor the progress of the patient with objective criteria and further optimize the application of the method.

Read more about the musculoskeletal ultrasound.


The results of Prolotherapy appear in two phases. Immediately after the treatment, it displays a direct effect, but the greater and permanent results appear after 4 to 8 weeks (in some cases may and 12 weeks) after the treatment. This is due to the way that the treatment works. Once again it is mentioned that in Prolotherapy we do not use pain killers, anti-inflammatory or foreign additives to correct the damaged tissue, but our treatment motivates the body to generate its own tissue and that takes time to be achieved.

Read more about prolotherapy in the following articles: